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Figure 8 | BMC Genomics

Figure 8

From: Fungi have three tetraspanin families with distinct functions

Figure 8

Pathogenicity of Δtsp3 and Δtpl1 deletion mutants from M. grisea. (A) Pathogenicity on barley. Barley cv. Plaisant leaves were inoculated with droplets of conidial suspension (3 × 104 conidia/ml) from pathogenic P1.2-Δku80::bar (+) and Δku80::bar/Δtsp3 and Δku80::bar/Δtpl1 mutants. Leaves were kept on water agar for 7 days under alternate day/night at 26°C and scored. Typical symptoms induced by M. grisea are visible as pale green ellipsoid lesions surrounded by a yellow halo for wild type as well as tetraspanin mutants showing that Δtsp3 and Δtpl1 mutants are still pathogenic on barley. (B) Pathogenicity on rice. Seedlings of rice cultivars Azucena and CO-39 were sprayed with conidial suspensions (2.5 × 104 conidia/ml) from pathogenic P1.2-Δku80::bar (+) and Δku80::bar/Δtsp3 and Δku80::bar/Δtpl1 mutants. Seedlings were incubated for 7 days under alternate day/night at 25°C for development of disease. The deletion mutants Δku80::bar/Δtsp3 and Δku80::bar/Δtpl1 produced significantly fewer disease lesions than P1.2-Δku80::bar (reference strain).

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