Figure 5From: A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundanceAnalysis of uniformity of amplification test. A. Ct frequency distribution. B. Correlation of Ct to total primer length, R: 0.08. C. Correlation of Ct to GC%, R: -0.12. D. Correlation of Ct to Tm, R: -0.29.Back to article page