A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance

Table 2 PrimerBank primer design and gene representation.

Mouse primer pair design	Number of mouse primer pairs or genes
Primer pairs with no redundancy	23700
Primer pairs with 2 target genes	2534
Primer pairs with more than 2 target genes	621
Total number of primer pairs	26855
Total number of genes represented	27684
Total number of genes not represented	1165

Primer pairs for the mouse genome have 27684 gene targets. Design constraints allowed only 26855 primer pairs to be synthesized, some of which amplify the same sequence from 2 genes or gene isoforms.

ISSN: 1471-2164