Figure 2

Gene regions & mixture complexity. (a, b) Signal plots (blue) for yeast ACT1 (a) and human HBG2 (b) genes, showing annotated coding regions (green). (Flanking regions not shown.) (c, d) Intensity normalization: Representative coding (left) and non-coding (right) regions selected from genes (including flanking regions) and used for normalization from ACT1 (c) and HBG2 (d). Median intensity in coding regions was set to 1, and median intensity in non-coding regions was set to 0 (red line). All slide intensities were normalized to these values. (e) Mixture complexity: Normalized intensity variance for ACT1 and HBG2. Increased mixture complexity in human corresponds to larger signal variance, particularly in low expressed regions.