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Table 2 Table of GC skew and equivalent substitutions

From: Mismatch oligonucleotides in human and yeast: guidelines for probe design on tiling microarrays

Substitution

T→G

C→A

T→C

G→A

A→G

C→T

A→C

G→T

C→G

T→A

G→C

A→T

Mispair

G-A

A-G

C-A

A-C

G-T

T-G

C-T

T-C

G-G

A-A

C-C

T-T

GC Effect

+

-

+

-

+

-

+

-

=

=

=

=

  1. All possible single nucleotide substitutions, grouped into equivalent mispairs (a): base substitution (top, shown as original→substitution) leads to mispair (middle row) with resulting effect on GC content of oligo (bottom, + for increase in oligo GC content, - for decrease).