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Table 2 Summary of primers used for library screening and expression analysis. BAC clone addresses are also given for each BAC clone identified and used in the localisation of the MRX genes.

From: Origin and evolution of candidate mental retardation genes on the human X chromosome (MRX)

Gene Primer Pair Use Sequence BAC clone Product size genomic/cDNA AnnealĀ°C
RSK2 MdRSK2 (17)F1
MdRSK2 (17)R1
MeRSK2 (13)F1
MeRSK2 (20)R1
BAC isolation expression F1: 5'-TGAAAGGAGGTGAACTACTGGA-3'
R1: 5'-ACCCCTTGTGCATGAAGATA-3'
F1: 5'-AGCCTGCAACAGGTAGACCT-3'
R1: 5'-TTTGCAGTGTCTGAAACGGA-3'
VIA 73C9 118 bp
1.0 kb
50
55
ARX MdARX F5
MdARX R5
BAC isolation/expression F5: 5'-AGCTCTGAGGCTGAAAGCAA-3'
R5: 5'-TTTGATTAGCGATGGTGGTG-3'
VIA 37C12 150 bp 57
TSPAN7 MeTSPAN7 OVa
MeTSPAN7 OVb
MdTM4SF2 F2
MdTM4SF2 R2
BAC isolation expression 5'-TGCCATGTTCTTGTCCCTGGTGTT-3'
5'-CACCAGCTCAGCCAAGAACACCAG-3'
F2: 5'-GAGCTGCTGTGGTGTTCAGA-3'
R2: 5'-CAAACCTTTTGATTGACTTTAGTGG-3'
AGI 383N14 40 bp
150 bp
n/a
58
SREB3 MdSREB3 F2
MdSREB3 R2
BAC isolation/expression F2: 5'-CTTGCACAAGGCTCCCTACT-3'
R2: 5'-TCATGCGTTTGGCATAGAAG-3'
AGI 63D15* 270 bp 52
JARID1C MeSMCX F1
MeSMCX R1
BAC isolation/expression F1: 5'-CGTCTGAGGAGGAGCCATAG-3'
R1: 5'-CCTTGAAGGAGTCAGCCATC-3'
AGI 63D15* 250 bp 57
OPHN1 MdOPHN1 F2
MdOPHN1 R2
BAC isolation/expression F2: 5'-CACAGCCGAGAGAACCTGAT-3'
R2: 5'-GGATTTCCACGACGATGTTC-3'
AGI 62P8 118 bp 59
RSK4 MeRSK4 F2
MeRSK4 R2
MeRSK4 (13)F1
MeRSK4 (20)R1
BAC isolation expression F2: 5'-CATTCAAACCTGCATCTGGA-3'
R2: 5'-TCGCTTGCAAACAGAATAGG
F1: 5'-AACCTGCATCTGGAAAACCA-3'
R1: 5'-TTTGCTGCATCTGAAACAGT-3'
AGI 88I19 650 bp
1.0 kb
60
55
FMR1 MdFMR1 OVa
MdFMR1 OVb
MeFMR1 F1
MeFMR1 R1
BAC isolation expression 5'-TGGTGCTAATAATCAGCAAGCTAG-3'
5'-GACTCCAGGTACTTTTCTAGCTTG-3'
F1: 5'-GACAGCATCACCAATGCAAC-3'
R1: 5'-CGCTGACGATTATCTGTTCG-3'
AGI 77O12 40 bp
450 bp
n/a
59
FMR2 MdFMR2 F1
MdFMR2 R1
MeFMR2 F2
MeFMR2 R2
BAC isolation expression F1: 5'-CTGCTAGCCATGTCAACATCA-3'
R1: 5'-TCAGTCTGTCTGCCATGTCC-3'
F2: 5'-AGAGCCTCCTTCAACCAACA-3'
R2: 5'-CCTGTTCTGGGTGTTCCAGT-3'
AGI 39E1 80 bp
450 bp
59
60
AGTR2 MeAGTR2 F1
MeAGTR2 R1
BAC isolation/expression F1: 5'-TACCAAGCCGTTGTCTACCC-3'
R1: 5'-CTGCGGAGCTGAGAAACTCT-3'
AGI 91P8 600 bp/650 bp 59
ARHGEF6 MeARHGEF6 F1
MeARHGEF6 R1
BAC isolation/expression F1: 5'-TTGGGCAACTTTGAGGAAGT-3'
R1: 5'-CATTCACAGCTGAGGGATGA-3'
AGI 139J23 2.0 kb/170 bp 57
  1. * Delbridge et al, in preparation.