Table 2 Summary of primers used for library screening and expression analysis. BAC clone addresses are also given for each BAC clone identified and used in the localisation of the MRX genes.
From: Origin and evolution of candidate mental retardation genes on the human X chromosome (MRX)
Gene | Primer Pair | Use | Sequence | BAC clone | Product size genomic/cDNA | Anneal°C |
|---|---|---|---|---|---|---|
RSK2 | MdRSK2 (17)F1 MdRSK2 (17)R1 MeRSK2 (13)F1 MeRSK2 (20)R1 | BAC isolation expression | F1: 5'-TGAAAGGAGGTGAACTACTGGA-3' R1: 5'-ACCCCTTGTGCATGAAGATA-3' F1: 5'-AGCCTGCAACAGGTAGACCT-3' R1: 5'-TTTGCAGTGTCTGAAACGGA-3' | VIA 73C9 | 118 bp 1.0 kb | 50 55 |
ARX | MdARX F5 MdARX R5 | BAC isolation/expression | F5: 5'-AGCTCTGAGGCTGAAAGCAA-3' R5: 5'-TTTGATTAGCGATGGTGGTG-3' | VIA 37C12 | 150 bp | 57 |
TSPAN7 | MeTSPAN7 OVa MeTSPAN7 OVb MdTM4SF2 F2 MdTM4SF2 R2 | BAC isolation expression | 5'-TGCCATGTTCTTGTCCCTGGTGTT-3' 5'-CACCAGCTCAGCCAAGAACACCAG-3' F2: 5'-GAGCTGCTGTGGTGTTCAGA-3' R2: 5'-CAAACCTTTTGATTGACTTTAGTGG-3' | AGI 383N14 | 40 bp 150 bp | n/a 58 |
SREB3 | MdSREB3 F2 MdSREB3 R2 | BAC isolation/expression | F2: 5'-CTTGCACAAGGCTCCCTACT-3' R2: 5'-TCATGCGTTTGGCATAGAAG-3' | AGI 63D15* | 270 bp | 52 |
JARID1C | MeSMCX F1 MeSMCX R1 | BAC isolation/expression | F1: 5'-CGTCTGAGGAGGAGCCATAG-3' R1: 5'-CCTTGAAGGAGTCAGCCATC-3' | AGI 63D15* | 250 bp | 57 |
OPHN1 | MdOPHN1 F2 MdOPHN1 R2 | BAC isolation/expression | F2: 5'-CACAGCCGAGAGAACCTGAT-3' R2: 5'-GGATTTCCACGACGATGTTC-3' | AGI 62P8 | 118 bp | 59 |
RSK4 | MeRSK4 F2 MeRSK4 R2 MeRSK4 (13)F1 MeRSK4 (20)R1 | BAC isolation expression | F2: 5'-CATTCAAACCTGCATCTGGA-3' R2: 5'-TCGCTTGCAAACAGAATAGG F1: 5'-AACCTGCATCTGGAAAACCA-3' R1: 5'-TTTGCTGCATCTGAAACAGT-3' | AGI 88I19 | 650 bp 1.0 kb | 60 55 |
FMR1 | MdFMR1 OVa MdFMR1 OVb MeFMR1 F1 MeFMR1 R1 | BAC isolation expression | 5'-TGGTGCTAATAATCAGCAAGCTAG-3' 5'-GACTCCAGGTACTTTTCTAGCTTG-3' F1: 5'-GACAGCATCACCAATGCAAC-3' R1: 5'-CGCTGACGATTATCTGTTCG-3' | AGI 77O12 | 40 bp 450 bp | n/a 59 |
FMR2 | MdFMR2 F1 MdFMR2 R1 MeFMR2 F2 MeFMR2 R2 | BAC isolation expression | F1: 5'-CTGCTAGCCATGTCAACATCA-3' R1: 5'-TCAGTCTGTCTGCCATGTCC-3' F2: 5'-AGAGCCTCCTTCAACCAACA-3' R2: 5'-CCTGTTCTGGGTGTTCCAGT-3' | AGI 39E1 | 80 bp 450 bp | 59 60 |
AGTR2 | MeAGTR2 F1 MeAGTR2 R1 | BAC isolation/expression | F1: 5'-TACCAAGCCGTTGTCTACCC-3' R1: 5'-CTGCGGAGCTGAGAAACTCT-3' | AGI 91P8 | 600 bp/650 bp | 59 |
ARHGEF6 | MeARHGEF6 F1 MeARHGEF6 R1 | BAC isolation/expression | F1: 5'-TTGGGCAACTTTGAGGAAGT-3' R1: 5'-CATTCACAGCTGAGGGATGA-3' | AGI 139J23 | 2.0 kb/170 bp | 57 |