Validation of severe expression changes detected by expression profiling. For six of the genes with the greatest alteration in gene expression changes, the expression levels were validated in two tissue types from three different wild type and three different DpDf sibling plants by semi-quantitative RT-PCR. L16798, BM380426, BM269210 and AI783234 are examples of increased expression in DpDf plants while AY107007 and BM073880 are examples of decreased expression in DpDf plants. Mez1 is used as a loading control. The first lane is a genomic DNA control while the last lane is a no template control (NTC).