Profiling expression changes caused by a segmental aneuploid in maize

BMC Genomics

Table 3 Analysis of genes with greater than 2-fold change between DpDf and wild type siblings

Accession	Wild type (signal)	DpDf (signal)	Fold change (DpDf/Wild type)	Expression validation^a	Chromosome^b	Annotation
Increased transcript levels in DpDf relative to wild type
AI666121	18	414	23.09	Present/absent	5	peptidyl-prolyl cis-trans isomerase
L16798	12	140	11.44	Present/absent	5	class I acidic chitinase
BM380426	31	339	11.10	Present/absent	1	unknown
AI783234	169	429	2.53	Semi-quantitative	5	unknown
BM269210	81	198	2.46	Present/absent	5	splicing factor Prp18
AY639019	49	119	2.41	Present/absent	8	phosphate transporter
AY105653	358	842	2.35	Semi-quantitative	5	malate oxidoreductase
CK369759	63	145	2.32	Present/absent	5	unknown
CF629635	187	375	2.01	Semi-quantitative	5	SEC14 cytosolic factor
AY107589	317	633	2.00	Semi-quantitative	5	unknown
U17897	200	415	2.07	NT^c	NT^c	starch branching enzyme I (sbe1)
Decreased transcript levels in DpDf relative to wild type
AY107007	78	37	0.47	Present/absent	6	unknown
AI622092	200	92	0.46	Present/absent	6	unknown
BG873775	135	62	0.46	Present/absent	6	lipid transfer protein
BM073880	109	48	0.44	Present/absent	6	similar to GCN5-like protein 1
AI737202	192	76	0.40	Present/absent	6	unknown
AI677337	874	427	0.49	NT^c	NT^c	unknown

^aExpression validation was assessed as "Present/absent" when amplicons were only detected in either DpDf or wild-type. Semi-quanitative indicates that expression was detected in both DpDf and wildtype with semi-quantitative variation in agreement with the microarray results.
^bChromosome positions determined from oat × maize chromosome addition lines (Kynast et al., 2001).
^cNot tested due to failed amplification

ISSN: 1471-2164