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Figure 5 | BMC Genomics

Figure 5

From: Human glycolipid transfer protein (GLTP) genes: organization, transcriptional status and evolution

Figure 5

Heterologous Expression and Glycolipid Intermembrane Transfer Activity of Human GLTP Homologs. A. Heterologous expression of GLTP homologs in E. coli using pET-30 vector shown by SDS-PAGE. Arrow shows migration position of 6xHis-S-GLTP homologs (~25 kDa). GLTP = left panel; Intronless GLTP (GLTPi) = right panel; Lane 1 = soluble fraction; Lane 2 = Inclusion body pellet. Std = molecular weight standards. B. Intermembrane glycolipid transfer activity of GLTP homologs. [3H]GalCer transfer measured as described in Methods and Materials. The bars represent the averages of 6 assays performed using 1.0 μg of GLTP homolog.

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