Figure 1
From: Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping
Amplification of SSRs from six bread wheat varieties in uniplex reactions using conventional and multiplex-ready PCR. Markers from left to right are gwm301, gwm340, gwm389 and gwm513. Multiplex-ready PCR assays were performed using 80, 70, 40 and 40 nM of locus-specific primer, respectively. Conventional PCR was performed with Ta 55, 60, 60 and 60°C, respectively. PCR products were separated on a GelScan2000 instrument.