Figure 4
From: Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping
Six-plex PCRs performed in duplicate for six apricot varieties. Multiplex-ready PCR for panel 1 was performed using SSR markers (A) UDP96-003, (B) UDP96-008, (C) BPPCT028, (D) BPPCT014, (E) pchgms21.2 and (F) pchgms17 with 50, 50, 40, 40, 70 and 50 nM of locus-specific primer, respectively; and for panel 2 using SSR markers (G) UDP96-010, (H) BPPCT039, (I) BPPCT004, (J) pchcms04, (K) pchgms11.2 and (L) pchgms23 with 60, 50, 50, 70, 70 and 70 nM of locus-specific primer, respectively. The SSR products were separated on a Gel Scan 2000 instrument.