Table 1 Primer names, sequences and annealing temperatures used for the characterization of barramundi MSTN-1 gene
Primer name | Sequence (5'-3') | Annealing temperature (°C) |
|---|---|---|
Myo-up1 | ATG CAT CYG TCT CAG ATT GTG [21] | 52 |
Myo-up2 | ATG AGC AYG CCA TCA CGG AGA | 52 |
Myo-up3 | CAA CTG GGG CAT CSA GAT TAA C | 52 |
Myo-up5 | GCT ACA AGG CCA ACT ATT GC | 52 |
Myo-up8 | ACA TAC AAC CTA TTA CAC | 45 |
Myo-pro | CCA CAC GAT GGC RCT RTC | 55 |
Jcu-R | GTT CAG TGG CCA TCA TCA T | 52 |
Myo-L1 | TTT CCC CTC GAA TCG AAG GC | 52 |
Myo-L3 | GCA ATA GTT GGC CTT GTA GCG | 52 |
Myo-L5 | TGA GGA TTC CTG GTT TCA CTC | 52 |
Myo-L8 | TGC TGG TGC GTT TCT TGG TC | 55 |
Myo-Utr | GGC ATA TGT ATA CAA TAC | 45 |
18S-F | TGGTTAATTCCGATAACGAACGA | 54-45 step-down |
18S-R | CGCCACCTGTCCCTCTAAGAA | 54-45 step-down |