Figure 2

MiRNAs showing significant differences in levels as a function of daily time within the y w control group. (A, B) Y w or y w;;cyc⁰¹ flies were collected at the indicated times during the third LD cycle. LMW RNA was prepared from head extracts and subjected to miRNA microarray profiling. Shown are the miRNAs with statistically different levels as a function of time within the y w group but not within the y w;;cyc⁰¹ group. P values are indicated for each miRNA. (C, D) Canton-S flies were collected at the indicated times during either the third LD or the first day of DD. Total RNA was prepared from head extracts and subjected to quantitative RT-PCR (qRT-PCR). Results reflect the average of four replicates from two independent experiments. Error bars are S.E.M.