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Table 2 Accuracy of QSS on mixtures of plasmid DNA before corrections

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

 

Percentage of each VIT

Total (%)

Mix n°

VIT1

VIT2

VIT3

VIT4

VIT5

VIT6

 

1

 

74.8

   

22.2

97

2

  

78.02

28.5

  

106.52

3

  

19.4

 

71.1

 

90.5

4

50

 

23.5

  

29.6

103.1

5

27.3

29.1

54.6

   

111

6

27.8

  

52.9

28.1

 

108.8

7

  

23.3

23.4

51.7

 

98.4

8

 

22.7

  

21.6

46.2

90.5

9

17

16.9

16.6

16.8

16.7

16.5

100.5

10

30.3

   

32.1

34.6

97

11

 

36.4

40.1

33.5

  

110

12

  

36.5

 

59.8

 

96.3

13

 

39.2

   

58.3

97.5

14

66.8

  

53.1

  

119.9

  1. Marker frequency quantification, obtained from the standard curves shown in Figure 2. The sum of frequencies in each mix is shown on the right.
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