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Table 2 Accuracy of QSS on mixtures of plasmid DNA before corrections

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

  Percentage of each VIT Total (%)
Mix n° VIT1 VIT2 VIT3 VIT4 VIT5 VIT6  
1   74.8     22.2 97
2    78.02 28.5    106.52
3    19.4   71.1   90.5
4 50   23.5    29.6 103.1
5 27.3 29.1 54.6     111
6 27.8    52.9 28.1   108.8
7    23.3 23.4 51.7   98.4
8   22.7    21.6 46.2 90.5
9 17 16.9 16.6 16.8 16.7 16.5 100.5
10 30.3     32.1 34.6 97
11   36.4 40.1 33.5    110
12    36.5   59.8   96.3
13   39.2     58.3 97.5
14 66.8    53.1    119.9
  1. Marker frequency quantification, obtained from the standard curves shown in Figure 2. The sum of frequencies in each mix is shown on the right.