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Table 3 Accuracy of QSS on mixtures of plasmid DNA after final corrections

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

  Percentage of each VIT Total (%)
Mix n° VIT1 VIT2 VIT3 VIT4 VIT5 VIT6  
1   77.1     22.9 100
2    73.3 26.7    100
3    21.4   78.6   100
4 48.5   22.8    28.7 100
5 24.6 26.2 49.2     100
6 25.6    48.6 25.8   100
7    23.7 23.7 52.6   100
8   25.1    23.9 51 100
9 16.9 16.8 16.6 16.7 16.6 16.4 100
10 31.2     33.1 35.7 100
11   33.1 36.4 30.5    100
12    37.9   62.1   100
13   40.2     59.8 100
14 55.7    44.3    100
  1. Frequency values after proportional correction to give a total of 100% in each mix.
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BMC Genomics

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