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Table 6 Sum of the frequency estimates of all the markers before final correction

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

Replicate n°

 

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

 

125

137

120

128

129

123

125

122

122

116

121

111

110

111

110

117

110

112

111

Total amount (%)

  1. The sum of the frequency estimates of all markers before final correction is given here as an indicator of the quality of the sequence traces. Two subsets of differing quality can be distinguished (see text): replicates 1–11 (sums range from 116–137%), replicates 12–19 (sums range from 111–117%).