Unrooted phylogenetic tree between rice, arabidopsis and wheat nsLTP gene families. The mature sequences of the 122 non-redundant wheat nsLTPs, the 49 rice nsLTPs, and the 45 arabidopsis nsLTPs were aligned using HMMalign and then manually refined. The phylogenetic tree was built from the protein alignment (Additional file 3) with the maximum-likelihood method using the PHYML program . When possible, subtrees including sequences of the same type are grouped and represented by a grey triangle close to which is indicated, in brackets, the number of sequences of arabidopsis, rice and wheat respectively. Subtrees are detailed in Figure 7. Bootstrap values (% of 100 re-sampled data set) are indicated for each node.