Table 1 Mean aRNA yields (μg) using the oligo-dT and ADP amplification methods from total RNA inputs of 500, 50 and 5 ng. Average yield was determined by spectrophotometric analysis from 2–4 replicate amplification reactions. The range of amplified RNA yields is also detailed.
Amp Method Yield (μg) | Amount of input RNA (ng) | ||
|---|---|---|---|
5 | 50 | 500 | |
Oligo-dT | 130 (+/- 12) | 178 (+/- 2) | 198 (+/- 35) |
ADP | - | 91 (+/- 2) | 149 (+/- 18) |