Characterization of ER binding sites identified only through xMAN probe mapping. Standard ChIP assays of ER were performed with anti-ER antibody. Immunoprecipitated DNA was quantified by qPCR using primers spanning 10 randomly selected regions identified by MAT only with xMAN probe mapping. The results are shown as vehicle (control, white bars) or estrogen (black bars) fold enrichment over input and are the average of three replicates±SE. The 10 regions are provided as NCBIv35 chromosomal coordinates: Site 1(chr10:94549192-94550690), Site 2(chr11:46253821-46255330), Site 3(chr11:100812061-100813479), Site 4(chr4:188058695-188060091), Site 5(chr5:52253648-52254807), Site 6(chr5:133383322-133384523), Site 7(chr7:150991115-150992368), Site 8(chr8:88996063-88997399), Site 9(chr8:99413611-99415262), Site 10(chr8:102555348-102556385).