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Table 2 BALBOA annotation of unclassified ORFs that are consistent over two or more datasets.

From: Extending bicluster analysis to annotate unclassified ORFs and predict novel functional modules using expression data

ORF

Predicted Function

Experimental Evidence (SGD)

Protein Sequence

YJR154W

Metabolism: Amino Acid (01.01)

Putative protein, unknown function. GFP-fusion protein localizes to cytoplasm.

Similarity (p = 2.8e-5) to cytosolic L-asparaginase (YDR321W).

YDL072C (YET3)

Energy: Respiration (02.13)

Null mutant has decreased level of secreted invertase (enables respiration of sucrose).

Human BAP31 homolog.

YGR149W

Energy: Respiration (02.13)

Putative protein, unknown function

Predicted integral membrane protein.

YCR072C (RSA4)

Transcription: rRNA (11.04.01)

Recently verified by MIPS – ribosomal biogenesis.

 

YDL167C (NRP1)

Transcription: rRNA (11.04.01)

Role in ribosome biogenesis and assembly (RCA).

 

YMR259C

Transcription: rRNA (11.04.01)/Transport Routes (20.09)

Putative protein, unknown function; GFP-fusion protein localizes to the cytoplasm.

 

YNL022C

Transcription: rRNA (11.04.01)

Putative protein of unknown function. GFP-fusion protein localizes to a single spot in the nucleus.

Similarity (p = 1.2e-18) to YNL061W involved in rRNA processing.

YDR361C (BCP1)

Transcription (11)/Ribosomal Proteins (12.01.01)

Associated with RPL23a & PL23b (Ribosomal sub-units) in Affinity Capture Expts.

 

YJL122W (ALB 1)

Transcription (11)/Ribosomal Proteins (12.01.01)

Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit.

 

YJR003C

Transcription: rRNA (11.04.01)/Transport Routes (20.09)

Putative protein. Detected in purified mitochondria. Role in ribosome biogenesis and assembly (RCA).

 

YLR196W (PWP1)

Ribosomal Proteins (12.01.01)/Transcription (11)

Protein with WD-40 repeats involved in rRNA processing.

 

YER049W (TPA1)

Ribosomal Proteins (12.01.01)/Cellular Transport (20)

Interacts with Sup45p (eRF1) and Sup35p (eRF3) and Pab1p; role in translation termination efficiency.

 

YDR282C

Transported Compounds(20.01)/Tansposable Elements (38)

Putative protein of unknown function.

Similarity (p = 2.2e-21) to YDL001W required for sporulation.

YGR266W

Transport Routes (20.09)

Protein of unknown function. Localizes to mitochondrial outer membrane and plasma membrane

Predicted to have single trans-membrane domain.

YIL039W

Transport Routes (20.09)

GFP-fusion localizes to the ER. Deletion confers sensitivity to GSAO (angiogenesis inhibitor drug).

 

YOR175C

Transport Routes (20.09)

Protein of unknown function. Co-purification with Ribosomes.

Member of MBOAT putative membrane bound O-acyltransferases.

YPL105C

Transport Routes (20.09)/Transcription (11)

Protein of unknown function. Co-purification with both Ribosomes & mitochondria.

 

YIL060W

Tansposable Elements (38)

Putative protein of unknown function. Mutant accumulates less glycogen than does wild type.

Similarity (p = 4.2e-06) to TyA & TyB Retrotransposons.

YJR030C

Tansposable Elements (38)

Putative protein of unknown function. Expression repressed in carbon limited cultures

Similar to YJL181w (cell cyle regulator) & MBP-1 binding site (cell cycle).

YIL157C

Biogenesis of Cellular Components: Mitochondrion (42.16)

Detected in Co-purified mitochondria. Null mutant is defective in cytochrome oxidase.

 

YML030W

Biogenesis of Cellular Components: Mitochondrion (42.16)

Putative protein of unknown function; GFP-fusion protein localizes to mitochondria.

 
  1. Unclassified ORFs consistently annotated over two or more datasets, additional labels in italics are from one dataset only. GFP = Green Fluorescent Protein; Rca = Reviewed Computational Analysis.