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Table 2 BALBOA annotation of unclassified ORFs that are consistent over two or more datasets.

From: Extending bicluster analysis to annotate unclassified ORFs and predict novel functional modules using expression data

ORF Predicted Function Experimental Evidence (SGD) Protein Sequence
YJR154W Metabolism: Amino Acid (01.01) Putative protein, unknown function. GFP-fusion protein localizes to cytoplasm. Similarity (p = 2.8e-5) to cytosolic L-asparaginase (YDR321W).
YDL072C (YET3) Energy: Respiration (02.13) Null mutant has decreased level of secreted invertase (enables respiration of sucrose). Human BAP31 homolog.
YGR149W Energy: Respiration (02.13) Putative protein, unknown function Predicted integral membrane protein.
YCR072C (RSA4) Transcription: rRNA (11.04.01) Recently verified by MIPS – ribosomal biogenesis.  
YDL167C (NRP1) Transcription: rRNA (11.04.01) Role in ribosome biogenesis and assembly (RCA).  
YMR259C Transcription: rRNA (11.04.01)/Transport Routes (20.09) Putative protein, unknown function; GFP-fusion protein localizes to the cytoplasm.  
YNL022C Transcription: rRNA (11.04.01) Putative protein of unknown function. GFP-fusion protein localizes to a single spot in the nucleus. Similarity (p = 1.2e-18) to YNL061W involved in rRNA processing.
YDR361C (BCP1) Transcription (11)/Ribosomal Proteins (12.01.01) Associated with RPL23a & PL23b (Ribosomal sub-units) in Affinity Capture Expts.  
YJL122W (ALB 1) Transcription (11)/Ribosomal Proteins (12.01.01) Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit.  
YJR003C Transcription: rRNA (11.04.01)/Transport Routes (20.09) Putative protein. Detected in purified mitochondria. Role in ribosome biogenesis and assembly (RCA).  
YLR196W (PWP1) Ribosomal Proteins (12.01.01)/Transcription (11) Protein with WD-40 repeats involved in rRNA processing.  
YER049W (TPA1) Ribosomal Proteins (12.01.01)/Cellular Transport (20) Interacts with Sup45p (eRF1) and Sup35p (eRF3) and Pab1p; role in translation termination efficiency.  
YDR282C Transported Compounds(20.01)/Tansposable Elements (38) Putative protein of unknown function. Similarity (p = 2.2e-21) to YDL001W required for sporulation.
YGR266W Transport Routes (20.09) Protein of unknown function. Localizes to mitochondrial outer membrane and plasma membrane Predicted to have single trans-membrane domain.
YIL039W Transport Routes (20.09) GFP-fusion localizes to the ER. Deletion confers sensitivity to GSAO (angiogenesis inhibitor drug).  
YOR175C Transport Routes (20.09) Protein of unknown function. Co-purification with Ribosomes. Member of MBOAT putative membrane bound O-acyltransferases.
YPL105C Transport Routes (20.09)/Transcription (11) Protein of unknown function. Co-purification with both Ribosomes & mitochondria.  
YIL060W Tansposable Elements (38) Putative protein of unknown function. Mutant accumulates less glycogen than does wild type. Similarity (p = 4.2e-06) to TyA & TyB Retrotransposons.
YJR030C Tansposable Elements (38) Putative protein of unknown function. Expression repressed in carbon limited cultures Similar to YJL181w (cell cyle regulator) & MBP-1 binding site (cell cycle).
YIL157C Biogenesis of Cellular Components: Mitochondrion (42.16) Detected in Co-purified mitochondria. Null mutant is defective in cytochrome oxidase.  
YML030W Biogenesis of Cellular Components: Mitochondrion (42.16) Putative protein of unknown function; GFP-fusion protein localizes to mitochondria.  
  1. Unclassified ORFs consistently annotated over two or more datasets, additional labels in italics are from one dataset only. GFP = Green Fluorescent Protein; Rca = Reviewed Computational Analysis.