Breeding and sampling design for the identification of heritable protein markers. A. Diagram illustrating our approach to identify specific proteins associated with HB behavior using mass spectrometry. B. Breeding design and origin of samples. Parallel breeding programs were conducted in Beaverlodge, Alberta (55°N, 119°W) and Grand Forks, British Columbia (49°N, 118°W). Samples for proteomic analysis were taken each year at each location. The partial diallel cross used with the instrumental insemination in AB and in Y3 in BC is shown in C.