RT-qPCR validation of the differentially regulated calcium channel genes at the epithelium (biological repeats ≥ 3). Cosine similarity-identified A)
CACNA1D and B)
ORAI1 were tested with different primer sets. Other high voltage-gated calcium channels C)
CACNA2D1 and E)
CACNA1G showed distinct expression patterns between feather and scale epithelium. *represents p-values < 0.05 for the bracketed comparisons.