Skip to main content

Table 2 Intra- and between method variation based on read abundance ratios*

From: Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

  Intra-method variation    Between method variation a
  FD1/FD2 DTT1/DTT2 CsCl1/CsCl2 MG1/MG2 FD/DTT DTT/CsCl CsCl/FD
Calculated with % read abundance  
M13 0.74 2.22 1.67 1.08 1.52 10.06 0.07
P22 0.96 1.01 1.01 1.73 0.95 0.78 1.36
ɸVPE25 0.94 0.92 0.75 2.33 0.99 125.23 0.01
T3 1.01 1.01 1.14 1.69 0.93 0.94 1.15
T7 1.03 1.00 0.71 1.88 0.94 1.57 0.68
Calculated with % read abundance normalized to genome size  
M13 0.75 2.24 1.63 0.73 1.60 11.20 0.06
P22 0.98 1.02 1.01 1.19 1.00 0.91 1.10
ɸVPE25 0.96 0.93 0.75 1.60 1.05 146.16 0.01
T3 1.03 1.01 1.14 1.16 0.98 1.09 0.93
T7 1.05 1.00 0.71 1.29 0.99 1.83 0.55
  1. *Perfect reproducibility would result in ratios equal to one.
  2. a:Read abundance ratios between methods were calculated using the average read abundance in each method.