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Table 3 Purification method biases for specific phages*

From: Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

  FD DTT CsCl MG
Average % read abundance normalized to genome size
M13 0.010 0.007 0.001 0.027
P22 79.605 79.695 87.830 29.338
ɸVPE25 5.564 5.310 0.036 9.749
T3 6.164 6.286 5.751 2.101
T7 4.879 4.923 2.691 2.974
Ratios of genome size normalized % read abundances
P22/ɸVPE25 14.3 15.0 2417.5 3.0
P22/T3 12.9 12.7 15.3 14.0
P22/T7 16.3 16.2 32.6 9.9
ɸVPE25/T3 0.9 0.8 0.0 4.6
ɸVPE25/T7 1.1 1.1 0.0 3.3
T3/T7 1.3 1.3 2.1 0.7
T7/M13 465 748 4584 108
P22/M13 7587 12119 149630 1073
ɸVPE25/M13 530 807 61 356
T3/M13 587 956 9797 76
  1. *In case of absence of purification biases, ratios of size normalized read abundances between phages would be identical in the unpurified control samples (MG) and the purified samples (FD, DTT and CsCl).