Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

Table 5 Assembly statistics

	Published sequence	FD1	FD2	DTT1	DTT2	CsCl1	CsCl2	MG1	MG2
P22 No. of contigs	1	1	1	1	1	1	1	1	1
P22 largest contig (bp)^a	41660	41737	41737	41737	41737	41737	41659	41737	41737
P22 coverage (x fold)	N/A	3651	3138	3925	4027	5489	4998	146	107
T7 No. of contigs	1	1	2	2	1	1	1	4	1
T7 largest contig (bp)	39937	39855	35301	35209	39344	39855	39855	35472	39855
T7 coverage (x fold)	N/A	256	220	257	273	168	202	16	11
T3 No. of contigs	1	1	4	4	2	1	1	2	1
T3 largest contig (bp)	38209	37540	33483	33392	37540	36121	37541	33656	36120
T3 coverage (x fold)	N/A	293	265	305	321	381	310	10	8
ɸVPE25 No. of contigs	1	1	1	1	1	1	1	1	2
ɸVPE25 largest contig (bp)	86524	86520	86522	86518	86542	86496	86505	86534	86540
ɸVPE25 coverage (x fold)	N/A	256	225	253	284	1.9	2.3	55	30
M13 No. of contigs	1	7	4	8	4	1	0	1	0
M13 largest contig (bp)	6407	1085	900	814	586	230	0	268	0
M13 coverage (x fold)	N/A	0.35	0.55	0.43	0.39	0.18	0	0.14	0

^a:Assemblies of the P22 genome from our datasets are slightly larger than the reference genome, which is likely due to the quasi-circular nature of the P22 genome, which makes it impossible for the assembly algorithm to determine the exact start and end of the genome.