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Table 3 Number of transcripts found in in vitro roots and leaves putatively encoding enzymes involved in withanolide biosynthesis

From: Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis

Enzymes Abbreviation Enzyme commission number No. of transcripts
Total Expressed in both adventitious roots and leaves Uniquely expressed in adventitious roots
1-Deoxy-D-xylulose-5-phosphate synthase DXPS 2.2.1.7 8 8 0
1-Deoxy-D-xylulose-5-phosphate reductoisomerase DXPR 1.1.1.267 3 3 0
2-C-Methyl-D-erythritol 4-phosphate cytidylyltransferase MEP-CT 2.7.7.60 2 2 0
4-(Cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase CDP-MEK 2.7.1.148 3 2 1
2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase MECDPS 4.6.1.12 1 1 0
(E)-4-Hydroxy-3-methylbut-2-enyl-diphosphate synthase HMBPPS 1.17.7.1 1 1 0
4-Hydroxy-3-methylbut-2-enyl diphosphate reductase HMBPPR 1.17.1.2 1 1 0
Isopentenyl-diphosphate delta-isomerase IDI 5.3.3.2 4 4 0
Acetyl-CoA C-acetyltransferase ACAT 2.3.1.9 19 12 7
Hydroxymethylglutaryl-CoA synthase HMGCS 2.3.3.10 5 5 0
Hydroxymethylglutaryl-CoA reductase HMGR 1.1.1.88 11 11 0
Hydroxymethylglutaryl-CoA reductase HMGR 1.1.1.34 9 9 0
Mevalonate kinase MK 2.7.1.36 3 3 0
Phosphomevalonatekinase PVMK 2.7.4.2 8 8 0
Diphosphomevalonate decarboxylase MVD 4.1.1.33 1 1 0
Geranyl-diphosphate synthase GPPS 2.5.1.1 38 35 3
Farnesyl-diphosphate synthase FPPS 2.5.1.10 19 19 0
Squalene synthase SS 2.5.1.21 5 5 0
Squalene monooxygenase SE 1.14.13.132 10 10 0
Cycloartenol synthase CAS 5.4.99.8 28 15 13
Sterol 24-C-methyltransferase SMT1 2.1.1.41 7 7 0
Methylsterol monooxygenase/Sterol-4a-methyl oxidase 2 SMO1/SMO2 1.14.13.72 7 6 1
Cycloeucalenol cycloisomerase CEC1 5.5.1.9 1 1 0
Obtusifoliol 14-demethylase CPY51G1 1.14.13.70 3 3 0
Delta 14-sterol reductase FK 1.3.1.70 3 3 0
C-7,8 Sterol isomerase HYD1 5.3.3.5 2 1 1
C-5 Sterol desaturase STE1 1.14.21.6 12 10 2
7-Dehydro cholesterol reductase DWF5 1.3.1.21 2 2 0
Sterol glycosyltransferases SGT 2.4.1.173 53 42 11
Methyltransferases MT   509 466 43