Skip to main content

Table 4 Antioxidant activity in methanolic extracts of adventitious root and leaves

From: Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis

Activity of antioxidants Tissue
In vitro adventitious root In vitro leaf
Enzymatic antioxidants (U/mg protein)
SOD 5.66 ± 0.35 3.38 ± 0.25
Peroxidase 3.02 ± 0.42 2.5 ± 0.57
GST 4.0 ± 0.25 2.4 ± 0.09
Polyphenol oxidase 3.50 ± 0.23 2.56 ± 0.35
Non-Enzymatic antioxidants (mg/g)
Vitamin A 3.54 ± 0.35 2.56 ± 0.25
Vitamin C 3.8 ± 0.27 1.47 ± 0.46
Vitamin E 10.2 ± 0.27 8.3 ± 0.57
Free radical scavenging activity (FRSA) 72.5 ± 0.58% 55 ± 0.47%
  1. The values of Mean ± SD of triplicates.
  2. SOD (U/mg): amount of SOD that causes 50% reduction in the extent of NBT oxidation.
  3. Peroxidase (U/mg): change in absorbance/min at 430 nm.
  4. GST (U/mg protein): moles of CDNB conjucated/min/mg of sample.
  5. Polyphenol oxidase: (U/mg protein): change in absorbance/minute at 495 nm.