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Table 4 Antioxidant activity in methanolic extracts of adventitious root and leaves

From: Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis

Activity of antioxidants

Tissue

In vitro adventitious root

In vitro leaf

Enzymatic antioxidants (U/mg protein)

SOD

5.66 ± 0.35

3.38 ± 0.25

Peroxidase

3.02 ± 0.42

2.5 ± 0.57

GST

4.0 ± 0.25

2.4 ± 0.09

Polyphenol oxidase

3.50 ± 0.23

2.56 ± 0.35

Non-Enzymatic antioxidants (mg/g)

Vitamin A

3.54 ± 0.35

2.56 ± 0.25

Vitamin C

3.8 ± 0.27

1.47 ± 0.46

Vitamin E

10.2 ± 0.27

8.3 ± 0.57

Free radical scavenging activity (FRSA)

72.5 ± 0.58%

55 ± 0.47%

  1. The values of Mean ± SD of triplicates.
  2. SOD (U/mg): amount of SOD that causes 50% reduction in the extent of NBT oxidation.
  3. Peroxidase (U/mg): change in absorbance/min at 430 nm.
  4. GST (U/mg protein): moles of CDNB conjucated/min/mg of sample.
  5. Polyphenol oxidase: (U/mg protein): change in absorbance/minute at 495 nm.
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BMC Genomics

ISSN: 1471-2164

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