Table 1 Summary of data generated from grass carp transcriptome
Summary | Â | A | B | C | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Brain | Gut | Liver | Brain | Gut | Liver | Brain | Gut | Liver |
Map to genome | Total Reads | 64914000 (100.00%) | 62679236 (100.00%) | 62801686 (100.00%) | 66466322 (100.00%) | 63096538 (100.00%) | 65442204 (100.00%) | 62196672 (100.00%) | 66338198 (100.00%) | 62079494 (100.00%) |
| Â | Total BasePairs | 5842260000 (100.00%) | 5641131240 (100.00%) | 5652151740 (100.00%) | 5981968980 (100.00%) | 5678688420 (100.00%) | 5889798360 (100.00%) | 5597700480 (100.00%) | 5970437820 (100.00%) | 5587154460 (100.00%) |
| Â | Total Mapped Reads | 49715390 (76.59%) | 42833150 (68.34%) | 42985997 (68.45%) | 50210581 (75.54%) | 43776373 (69.38%) | 43400816 (66.32%) | 47148316 (75.81%) | 45696711 (68.88%) | 43017860 (69.29%) |
| Â | Perfect match | 37846054 (58.30%) | 31528381 (50.30%) | 30706529 (48.89%) | 37491544 (56.41%) | 31800339 (50.40%) | 30572108 (46.72%) | 36533605 (58.74%) | 33008461 (49.76%) | 31336236 (50.48%) |
|  | ≤5 bp mismatch | 11869336 (18.28%) | 11304769 (18.04%) | 12279468 (19.55%) | 12719037 (19.14%) | 11976034 (18.98%) | 12828708 (19.60%) | 10614711 (17.07%) | 12688310 (19.13%) | 11681624 (18.82%) |
| Â | Unique match | 48342500 (74.47%) | 40897483 (65.25%) | 41471932 (66.04%) | 48691487 (73.26%) | 41959590 (66.50%) | 42291590 (64.62%) | 45938250 (73.86%) | 43731437 (65.92%) | 41795927 (67.33%) |
| Â | Multi-position match | 1372890 (2.11%) | 1935667 (3.09%) | 1514065 (2.41%) | 1519094 (2.29%) | 1816783 (2.88%) | 1109226 (1.69%) | 1210066 (1.95%) | 1965334 (2.96%) | 1221933 (1.97%) |
| Â | Total Unmapped Reads | 15198610 (23.41%) | 19846086 (31.66%) | 19815689 (31.55%) | 16255741 (24.46%) | 19320165 (30.62%) | 22041388 (33.68%) | 15048356 (24.19%) | 20641427 (31.12%) | 19061634 (30.71%) |
Map to gene | Total Reads | 64914000 (100.00%) | 62679236 (100.00%) | 62801686 (100.00%) | 66466322 (100.00%) | 63096538 (100.00%) | 65442204 (100.00%) | 62196672 (100.00%) | 66338198 (100.00%) | 62079494 (100.00%) |
| Â | Total BasePairs | 5842260000 (100.00%) | 5641131240 (100.00%) | 5652151740 (100.00%) | 5981968980 (100.00%) | 5678688420 (100.00%) | 5889798360 (100.00%) | 5597700480 (100.00%) | 5970437820 (100.00%) | 5587154460 (100.00%) |
| Â | Total Mapped Reads | 19866103 (30.60%) | 29656785 (47.32%) | 31817288 (50.66%) | 27628167 (41.57%) | 26090271 (41.35%) | 35023773 (53.52%) | 26422927 (42.48%) | 30446461 (45.90%) | 34294871 (55.24%) |
| Â | Perfect match | 15622780 (24.07%) | 22768765 (36.33%) | 24089872 (38.36%) | 21432825 (32.25%) | 19713788 (31.24%) | 26347072 (40.26%) | 21345319 (34.32%) | 22979918 (34.64%) | 26576843 (42.81%) |
|  | ≤4 bp mismatch | 4243323 (6.54%) | 6888020 (10.99%) | 7727416 (12.30%) | 6195342 (9.32%) | 6376483 (10.11%) | 8676701 (13.26%) | 5077608 (8.16%) | 7466543 (11.26%) | 7718028 (12.43%) |
| Â | Unique match | 19376349 (29.85%) | 28694650 (45.78%) | 30598309 (48.72%) | 26605613 (40.03%) | 25430799 (40.30%) | 34464752 (52.66%) | 25908582 (41.66%) | 29607406 (44.63%) | 33598282 (54.12%) |
| Â | Multi-position match | 489754 (0.75%) | 962135 (1.54%) | 1218979 (1.94%) | 1022554 (1.54%) | 659472 (1.05%) | 559021 (0.85%) | 514345 (0.83%) | 839055 (1.26%) | 696589 (1.12%) |
| Â | Total Unmapped Reads | 45047897 (69.40%) | 33022451 (52.68%) | 30984398 (49.34%) | 38838155 (58.43%) | 37006267 (58.65%) | 30418431 (46.48%) | 35773745 (57.52%) | 35891737 (54.10%) | 27784623 (44.76%) |