Figure 4

cDNA sequences support the re-annotation of gene11268. (A) F. vesca gene11268 annotation predicted by the first generation annotation at GDR. Colored boxes denote exons and gray lines denote introns. (B) Re-annotation of F. vesca gene11268 revealed the presence of additional exons. RNA-Seq reads from stage 7_8 anther are represented as red rectangles. Gray peaks below the red rectangles represent the abundance of additional reads beyond those shown. (C) The TowU_Fve predicted structure of F. vesca gene11268 after splicing is supported by the sequence of cDNA clones from YW5AF7 anther mRNA. Sequences of two such cDNA clones were identical and yielded the TowU_Fve predicted gene structure as shown.