Comparison of young/old fibroblast response to LPS as measured by RT-PCR. Comparison of gene expression following exposure to LPS at hours 0, 2, and 8 for young and old cultures. IL-8 (A), TNF-α (B), CD14 (C), and TLR4 (D) mRNA were all targeted. Values are expressed as dCt using β-actin expression as the endogenous control. Fold difference in expression between young and old fibroblasts presented for each time point within parentheses. All values are mean ± S.E.M. (n = 3/group). p-values are presented following analysis using a two-way repeated measures ANOVA.