The role of DNA methylation on gene expression and differential methylation levels due to genomic region. Scatter plot showing gene expression at 8 hours post-LPS exposure in relation to the level of DNA methylation at the gene’s (A) promoter or (B) within the gene itself. Expression levels for RNA-Seq and methylation data were normalized to gene size and presented as RPKM. Each value represents the average expression of the six young and old cultures investigated. Data obtained by RNA-Seq and MIRA-Seq. (C) Average methylation levels based upon genomic region for young/old MIRA-Seq libraries. Lettering denotes differential levels of methylation as measured by a one-way ANOVA with Bonferonni post-test (p < 0.05). All values are mean ± S.E.M.