Age dependent changes in the LPS induced transcriptome of bovine dermal fibroblasts occurs without major changes in the methylome

Table 2 Differential expression of immune associated genes at 0, 2, and 8 hours post-LPS exposure of fibroblasts collected at 16 months of age compared to fibroblasts collected at 5 months of age from the same three animals

Gene	Hour 0	Hour 2	Hour 8
Innate Immune Related Genes
CCL2	- -	7.3	3.2
CCL20	- -	18.1	10.1
CCL5	- -	10.7	3.4
CD14	5.5	5.7	4.2
IFIH1	- -	- -	2.1
IL1A	- -	2.3	2.8
IL6	- -	10.5	7.9
IL8	- -	5.8	3.3
MX2	- -	- -	33.9
OAS1	- -	- -	12.8
OAS2	- -	- -	10.6
SAA3	- -	- -	5.1
TLR2	- -	4	2.6
TLR4	2.1	- -	2.6
TNF	- -	10.4	- -
DNA Methylation Associated Genes
DNMT1	1.6	1.6	1.6
DNMT3A	- -	- -	- -
DNMT3B	- -	- -	- -
UHRF1	3.4	3.3	3.6

Data obtained by RNA-Seq and presented as fold change between cultures from younger and older animals. Positive values indicate higher expression in 16-month old cultures as compared to cultures from 5-month old animals. All presented values indicate FDR < 0.05 with an average CPM > 1, while - - notes a values with either FDR > 0.05 or an average CPM < 1.

ISSN: 1471-2164