Figure 5

Heat map of RNA-seq based expression data of putative reference genes, collected from 18 phylogenetically distant fungi. For each RNA-seq dataset (study), we calculated for each gene fold changes (FC) as the ratio between the expression in a condition of interest and the expression in the condition that was defined as the control in this study. Each line represents a condition of interest, each column a gene of interest (corresponding names of the genes are given in Figure 6). Genes have been distributed in three groups: the ‘R series’ that corresponds to 12 candidate reference genes pre-selected from T. versatilis data; the ‘C series’ that corresponds to more classic reference genes previously used in most of gene expression studies, including for filamentous fungi; and the ‘Sc series’ that corresponds to genes homologous to S. cerevisiae genes, which were previously validated as promising reference genes in this yeast species. Numbers reported in the heat map correspond to log _{(base 2)} of FC values. Colour scale and correspondences between Log _{(base 2)} and FC values are indicated in the legend (green colour set for a fold-change of 1 (log₂ = 0); red colour arbitrary set for differential expression equal or higher than 12 (|log₂| ≥ 3,5). Empty cells: data not available.