Figure 3

Correlation between DNA hypomethylation and gene expression of candidate genes. (A) Seven genes (ANGPT1, APBB2, CHGA, CTGF, IFI16, IGLON5, and SLC43A2) showed a correlation between their levels of DNA hypomethylation and gene expression. The methylation fold-change values were obtained from the HumanMethylation450K profile, and the gene expression fold-change values were quantified by qRT-PCR from HCT116 cells with either 5-aza-dC treatment (0.5 μM) or IR (2 or 5 Gy). The bar graphs indicate the gene expression level, and the line charts indicate the methylation level. (B) Quantitative MSP (qMSP) analysis of the CpG island of the APBB2, CHGA, and CTGF gene are shown. The promoter region (defined as upstream and downstream 1 kb from the transcriptional start site; TSS) includes typical CpG islands. The MSP primer location is indicated with a red bar. Each bar graph indicates the DNA methylation level of the APBB2, CHGA, and CTGF gene by qMSP, respectively. The candidate gene methylation values were normalized to the methylation of an Alu element. *P < 0.05; ***P < 0.001.