Effects of TIIA on cell growth and proliferation of AGS cells. (A) Growth curves show a dosage-dependent pattern of growth inhibition after TIIA treatment for 72 hr. All TIIA treatment conditions had significant effects on AGS cell growth (p < 0.0001, Wilcoxon Signed-Rank Test). Cell indexes of AGS cells were measured by an RTCA DP® system, expressed as mean ± SD of three replications. (B) Colonies of AGS cells were stained by crystal violet (purple-colored dots) after TIIA treatment. Control conditions exhibit many colonies, while IC50 TIIA conditions have produced almost no colonies. (C) TIIA significantly reduces the number of AGS colonies (*p < 0.05, **p < 0.01, Student’s t-Test) in a dosage-dependent manner. Histogram values are expressed as mean ± SD from three replications.