Figure 3

MS/MS spectra of peptide and protein levels for PSMB3, RS2 and G6PI. MS/MS spectra of peptides from PSMB3 (A), RS2 (B) and G6PI (C) are reported along with iTRAQ ion reporter quantification. Ion, m/z 114 and 115 represent peptides collected from control samples. Ion, m/z 116 and 117 represent peptides collected from TIIA-treated samples. (D) Protein expression levels of PSMB3, RS2 and G6PI were examined using western blotting analysis, with β-actin as an internal control. All experiments were repeated three times with independent samples.