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Table 2 List of iTRAQ-identified proteins regulated by TIIA

From: Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells

No. Biological function Accession Protein name Gene symbol Protein score No. of quantified peptides Coverage (%) iTRAQ ratio RNA-Seq ratio
1 Glycolysis P11166 Solute carrier family 2, facilitated glucose transporter member 1 SLC2A1 72 2 3 1.273 1.573
2 Glycolysis Q8N0Y7 Probable phosphoglycerate mutase 4 PGAM4 40 1 5.5 1.057 0.309
3 Glycolysis P06733 Alpha-enolase ENO1 720 15 46.8 0.947 0.754
4 Glycolysis P00338 L-lactate dehydrogenase A chain LDHA 283 6 29.2 0.901 0.352
5 Glycolysis P04406 Glyceraldehyde-3-phosphate dehydrogenase GAPDH 813 14 55.2 0.823 0.934
6 Glycolysis P07195 L-lactate dehydrogenase B chain LDHB 274 9 29 0.736 0.553
7 Glycolysis O60701 UDP-glucose 6-dehydrogenase UGDH 49 1 4.3 1.001 1.938
8 Glycolysis P06744 Glucose-6-phosphate isomerase GPI 83 1 7.3 0.525 0.824
9 ATP metabolic process P55072 Transitional endoplasmic reticulum ATPase VCP 129 4 7.3 0.888 0.661
10 ATP metabolic process P04075 Fructose-bisphosphate aldolase A ALDOA 196 5 30.5 0.804 1.284
11 Protein folding P14625 Endoplasmin HSP90B1 67 1 8.7 1.685 0.738
12 Protein folding P60709 Actin, cytoplasmic 1 ACTB 1161 30 47.2 1.129 0.795
13 Protein folding P62937 Peptidyl-prolyl cis-trans isomerase A PPIA 242 6 42.4 1.050 0.640
14 Protein folding P23284 Peptidyl-prolyl cis-trans isomerase B PPIB 69 2 11.6 1.084 0.988
15 Protein folding P10809 60 kDa heat shock protein, mitochondrial HSPD1 522 7 21.6 1.011 0.724
16 Protein folding P07900 Heat shock protein HSP 90-alpha HSP90AA1 614 7 27.7 0.991 0.572
17 Protein folding P38646 Stress-70 protein, mitochondrial HSPA9 71 1 4.1 1.037 0.946
18 Protein folding P11142 Heat shock cognate 71 kDa protein HSPA8 728 18 43.7 0.898 0.804
19 Protein folding P50991 T-complex protein 1 subunit delta CCT4 55 1 4.5 0.890 0.883
20 Protein folding P49368 T-complex protein 1 subunit gamma CCT3 56 1 4.8 1.119 0.876
21 Protein folding P50454 Serpin H1 SERPINH1 43 1 7.9 0.870 0.568
22 Protein folding Q99832 T-complex protein 1 subunit eta CCT7 38 1 7.7 0.896 0.812
23 Protein folding Q15084 Protein disulfide-isomerase A6 PDIA6 44 1 3.4 0.910 0.876
24 Protein folding P08238 Heat shock protein HSP 90-beta HSP90AB1 549 5 32.6 0.783 0.738
25 DNA damage response Q96QE3 ATPase family AAA domain-containing protein ATAD5 44 1 3.4 2.004 0.307
26 DNA damage response P49720 Proteasome subunit beta type-3 PSMB3 105 1 8.8 1.799 0.765
27 DNA damage response P62979 Ubiquitin-40S ribosomal protein S27a RS27A 46 1 21.2 1.115 NA
28 DNA damage response Q96QV6 Histone H2A type 1-A HIST1H2AA 205 1 40.5 1.006 NA
29 Cell proliferation Q06830 Peroxiredoxin-1 PRDX1 114 3 31.2 1.098 0.987
30 Cell proliferation P22392 Nucleoside diphosphate kinase B NME2 142 5 47.4 0.924 NA
31 Cell cycle P85299 Proline-rich protein 5 SMR3A 39 1 1.8 1.048 NA
32 G2/M transition of mitotic cell cycle P07437 Tubulin beta chain TUBB 660 5 31.8 0.927 0.508
33 Apoptotic process P02545 Prelamin-A/C LMNA 56 2 5.9 1.585 1.457
34 Apoptotic process P23528 Cofilin-1 CFL1 101 1 17.5 1.503 0.735
35 Apoptotic process P05783 Keratin, type I cytoskeletal 18 KRT18 350 8 36 1.327 2.884
36 Apoptotic process P04264 Keratin, type II cytoskeletal 1 KRT1 1877 38 10.7 1.088 NA
37 Apoptotic process O43707 Alpha-actinin-4 ACTN4 324 10 14.3 1.054 1.560
38 Apoptotic process P61978 Heterogeneous nuclear ribonucleoprotein K HNRNPK 221 5 16.6 0.938 0.760
39 Apoptotic process P30101 Protein disulfide-isomerase A3 PDIA3 40 1 9.7 0.702 0.889
40 Apoptotic process P11021 78 kDa glucose-regulated protein HSPA5 191 3 22.3 0.633 1.859
41 Angiogenesis P19338 Nucleolin NCL 242 6 20.4 1.242 0.504
42 Angiogenesis P07355 Annexin A2 ANXA2 412 14 51.9 1.217 1.692
43 Cytoskeleton organization P15311 Ezrin EZR 129 4 17.6 1.377 2.328
44 Cytoskeleton organization P08727 Keratin, type I cytoskeletal 19 KRT19 404 3 39.3 1.329 2.486
45 Cytoskeleton organization P05787 Keratin, type II cytoskeletal 8 KRT8 813 19 52 1.317 2.486
46 Cytoskeleton organization P07737 Profilin-1 PFN1 281 5 34.3 0.923 0.696
47 Actin crosslink formation P21333 Filamin-A FLNA 64 1 2.2 1.055 1.644
48 Microtubule cytoskeleton organization Q9BQE3 Tubulin alpha-1C chain TUBA1C 1208 23 45.7 0.869 0.717
49 DNA repair P06748 Nucleophosmin NPM1 145 3 21.1 0.973 0.785
50 DNA ligation P12956 X-ray repair cross-complementing protein 6 XRCC6 76 2 4.4 0.841 0.746
51 DNA repair Q13263 Transcription intermediary factor 1-beta TRIM28 68 2 4.8 1.021 0.841
52 DNA replication P55209 Nucleosome assembly protein 1-like 1 NAP1L1 61 1 7.4 0.913 0.895
53 DNA replication Q01105 Protein SET SET 77 1 3.4 0.826 0.613
54 Nucleosome assembly P04908 Histone H2A type 1-C HIST1H2AB 175 2 37.7 1.166 NA
55 Nucleosome assembly Q99879 Histone H2B type 1-M HIST1H2BM 355 9 55.6 1.073 NA
56 Nucleosome assembly Q16695 Histone H3.1 t HIST3H3 95 5 35.3 0.991 NA
57 Nucleosome assembly P62805 Histone H4 HIST2H4A 406 14 51.5 0.991 NA
58 Translational elongation P46783 40S ribosomal protein S10 RPS10 82 2 5.5 1.862 1.066
59 Translational elongation P46777 60S ribosomal protein L5 RPL5 36 1 4.7 1.389 1.106
60 Translational elongation P08865 40S ribosomal protein SA RPSA 61 1 14.6 1.243 1.296
61 Translational elongation P62277 40S ribosomal protein S13 RPS13 95 2 23.2 1.014 1.479
62 Translational elongation P61313 60S ribosomal protein L15 RPL15 53 1 5.9 0.983 1.129
63 Translational elongation P32969 60S ribosomal protein L9 RPL9 78 1 18.8 0.838 1.508
64 Translational elongation P23396 40S ribosomal protein S3 RPS3 69 3 23.9 0.845 1.240
65 Translational elongation P13639 Elongation factor 2 EEF2 451 7 24.1 0.796 2.148
66 Translational elongation P62249 40S ribosomal protein S16 RPS16 65 1 25.3 1.042 1.397
67 Translational elongation P50914 60S ribosomal protein L14 RPL14 80 1 25.6 0.744 1.316
68 Translational elongation P39019 40S ribosomal protein S19 RPS19 76 2 31 0.702 1.710
69 Translational elongation Q02543 60S ribosomal protein L18a RPL18A 50 1 5.1 0.709 1.369
70 Translational elongation P46778 60S ribosomal protein L21 RPL21 58 1 9.4 0.609 1.377
71 Translational elongation P18124 60S ribosomal protein L7 RPL7 58 1 11.3 0.538 1.304
72 Translational elongation P15880 40S ribosomal protein S2 RPS2 38 1 11.3 0.333 1.370
73 RNA metabolic process P55010 Eukaryotic translation initiation factor 5 EIF5 51 1 3.5 2.675 1.248
74 RNA metabolic process P07910 Heterogeneous nuclear ribonucleoproteins C1/C2 HNRNPC 158 2 20.3 0.917 0.682
75 RNA metabolic process O14979 Heterogeneous nuclear ribonucleoprotein D-like HNRNPDL 63 2 6.4 0.810 NA
76 RNA metabolic process Q32P51 Heterogeneous nuclear ribonucleoprotein A1-like 2 HNRNPA1L2 250 7 29.4 0.764 1.117
77 RNA metabolic process P60842 Eukaryotic initiation factor 4A-I EIF4A1 115 2 16.3 0.725 0.595
78 RNA metabolic process P52597 Heterogeneous nuclear ribonucleoprotein F HNRNPF 116 2 9.6 0.647 0.699
79 Epidermis development P02533 Keratin, type I cytoskeletal 14 KRT14 266 1 21.4 2.455 0.667
80 Epidermis development P35527 Keratin, type I cytoskeletal 9 KRT9 506 16 26.6 1.072 NA
81 Ectoderm development P04259 Keratin, type II cytoskeletal 6B KRT6B 432 1 23 1.225 22.537
82 Sulfur amino acid metabolic process P23526 Adenosylhomocysteinase AHCY 43 1 9.7 1.798 0.967
83 Epithelial to mesenchymal transition Q99729 Heterogeneous nuclear ribonucleoprotein A/B HNRNPAB 62 1 2.4 1.640 0.543
84 Energy reserve metabolic process P05141 ADP/ATP translocase 2 SLC25A5 58 1 5.7 1.412 1.234
85 Embryo development P60174 Triosephosphate isomerase TPI1 63 1 4.5 1.362 0.636
86 NADH metabolic process P40926 Malate dehydrogenase, mitochondrial MDH2 46 1 3.3 1.262 1.022
87 GTP catabolic process P68371 Tubulin beta-4B chain TUBB4B 490 2 31 1.216 0.572
88 Cellular membrane organization P63104 14-3-3 protein zeta/delta YWHAZ 224 2 34.3 1.208 1.016
89 Muscle contraction P07951 Tropomyosin beta chain TPM2 44 1 16.2 1.418 0.744
90 Activation of MAPKK activity P31946 14-3-3 protein beta/alpha YWHAB 188 2 24.8 1.201 0.616
91 Protein export from nucleus P63241 Eukaryotic translation initiation factor 5A-1 EIF5AL1 54 1 7.8 1.105 0.772
92 Keratinization P35908 Keratin, type II cytoskeletal 2 epidermal KRT2 1260 21 38.2 0.974 NA
93 Cellular response to calcium ion P13645 Keratin, type I cytoskeletal 10 KRT10 1903 29 45 0.963 1.831
94 Response to hypoxia P14618 Pyruvate kinase PKM PKM 352 10 26.9 0.918 0.811
95 RNA transport P22626 Heterogeneous nuclear ribonucleoproteins A2/B1 HNRNPA2B1 199 4 18.7 0.881 0.281
96 Histone mRNA metabolic process P62318 Small nuclear ribonucleoprotein Sm D3 SNRPD3 45 1 11.1 0.931 0.479
97 Positive regulation of protein phosphorylation P63244 Guanine nucleotide-binding protein subunit beta-2-like 1 GNB2L1 68 1 7.9 0.860 1.189
98 GTP catabolic process P68104 Elongation factor 1-alpha 1 EEF1A1 543 18 34 0.859 1.596
99 Response to virus P26641 Elongation factor 1-gamma EEF1G 195 4 9.6 0.763 1.257
100 Cilium assembly Q15051 IQ calmodulin-binding motif-containing protein 1 IQCB1 41 1 2 0.759 1.429
101 Cell redox homeostasis P07237 Protein disulfide-isomerase P4HB 86 3 7.5 0.710 1.076
102 RNA binding Q92804 TATA-binding protein-associated factor 2 N TAF15 56 1 2.9 0.614 0.372