Representative images of non-treated preadipocytes (1A), preadipocytes treated with DMIOA (1B), showing remarkable lipid accumulation in preadipocytes treated with DMIOA and relative expressions of FABP4 and C/EBPB in control and DMIOA treated cells (1C). Preadipocytes were cultured in Dulbecco’s modified eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) for 96 hr. Images were taken using an EVOS® xl core cell culture microscope (Advanced Microscopy Group, Seattle, USA) at 20X magnification. Green arrows indicate lipid droplets stained with Oil red O stain whereas there is no lipid formation in non-treated cells. C: Fold change expression of FABP4 and C/EBPβ in preadipocytes treated with an adipogenic cocktail (DMIOA) compared with non-treated cells. Preadipocytes were cultured in Dulbecco’s modified eagle’s medium containing 10% Fetal Bovine Serum for 96 hr. Bars with different letters are significantly different (P < 0.05). The bars represent Mean ± SD (N = 3).