Figure 6

The top significantly changed GO terms (molecular and cellular functions) with the proportion of genes involved among these over-expressed in cells treated with an adipogenic cocktail (DMIOA) (black bars) and those treated with a combination of DMIOA and 5 μM 20(S) (white bars) relative to each other. Lists of genes over-expressed in a given treatment group relative to the other were imported into IPA and the number of genes that were associated with a given GO term in the IPA data base was determined at P ≤ 0.0001. The P-value, calculated using the right-tailed Fisher Exact Test, is a measure of the likelihood that the association between the number of focus genes in the data set and a given GO term is due to random chance. The smaller the P-value is the less likely that the association is random and the more significant the association is. The p-value for a given GO term was calculated by considering the number of focus genes that participate in a given GO term and the total number of genes that are known to be associated with that GO term in the Ingenuity Knowledge Base. Then, the proportion of genes in a given GO term was calculated by dividing the number of genes determined by IPA that are associated with a given GO term in the Ingenuity® Knowledge Base by the total number of genes over-expressed in one treatment group relative to the other multiplied by 100.