Transcriptome analysis of hen preadipocytes treated with an adipogenic cocktail (DMIOA) with or without 20(S)-hydroxylcholesterol

Table 1 List of primers used for quantitative real-time polymerase chain reaction

Name	Forward	Reverse	Product length (base pair)	Annealing temperature (°C)
FABP4	GAGTTTGATGAGACCACAGCAGA	ATAACAGTCTCTTTGCCATCCCA	106	57
IGFBP7	TCCATCGTGACCCCTCCTAA	GAGAGATCAGTACCCAGCCG	228	55
VEGFc	AAGTGTGTGTGGATGTGGGG	TGACAGTTACGGGTTTGGGG	201	55
CD44	CGCTGTGCGGAGATACAGAA	CCTATGGCTCTTCCTGGCTG	199	55
MMP1	GCAGTCTCCTCTGCTTTCCC	GTCACGGTCAGGTTTCCCAG	209	58
STARD4	GGGACAGCACAAGCCCTAAT	GCCTAGCTTGACTGGGTTCA	219	54
INSIG1	GCATGGTGCCAGTGTGAAAG	TCCAGAGAACAGCCATACGC	222	54
GAPDH	GCTAAGGCTGTGGGGAAAGT	TCAGCAGCAGCCTTCACTAC	116	55
TGM4	TGGATGTCCTCTGACTCCGT	CAGTAGACCTTGTCGGCGTT	238	54
CCL4	CTCATGGCAGGTGCTGTTTG	CCTCCCTTAAATGCCCTCCC	207	55
TNFAIP3	CAGAAAAGAGGCCTGCTCCA	CCTTCAGTTTCTCGGGTGCT	202	54
GSTA3	GCCAAAGGAAACCACGCCTA	GTTTCATCCAGTGTACCGCCT	218	55
APOA1	CTCGCTGTGCTCTTCCTGAC	GTCAGCCAGCTTCAGGTCAA	191	55
KLF2	CTTACCCGCCACTACCGAAA	TTGTCCGGCTCTGTCCTAAG	123	58
HAS2	CACTGGGAGAAGCGTGGAAT	GCACTGTACGCAGCCAAAAT	203	56

ISSN: 1471-2164