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Table 2 Fold change and probability values of qRT-PCR and micro array data

From: Transcriptome analysis of hen preadipocytes treated with an adipogenic cocktail (DMIOA) with or without 20(S)-hydroxylcholesterol

Gene name

Fold change

P value

 

RT-PCR

Micro array

RT-PCR

Micro array

CD44

8.2

5.5

P = 0.02

P = 0.011

IGFBP7

12.1

9.1

P = 0.0002

P = 0.046

VEGFc

4.1

8.5

P = 0.0001

P = 0.027

MMP1

367

598.0

P = 0.001

P = 0.001

APOA1

61.0

12.2

P = 0.0009

P = 0.021

STARD4

4.0

6.2

P = 0.0008

P = 0.043

INSIG1

6.8

6.9

P = 0.0005

P = 0.012

TGM4

4.5

4.2

P =0.0004

P = 0.032

TNFAIP3

4.6

9.7

P = 0.001

P = 0.008

GSTA3

1.4

5.9

P = 0.04

P = 0.049

KLF2

6.5

3.9

P = 0.001

P = 0.04

HAS2

0.8

6.2

P = 0.15

P = 0.00048

  1. CD44, IGFBP7, and VEGFc were genes over expressed in control cells and MMP1 and APOA1 were genes over expressed in cells treated with DMIOA relative to each other. Similarly, STARD4, INSIG1, and TGM4 were genes over expressed in DMIOA treated cells whereas TNFAIP3, GSTA3, and KLF2 were genes over expressed in cells treated with DMIOA + 20(S) relative to each other.
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BMC Genomics

ISSN: 1471-2164

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