Figure 6

Segregation of the mutant phenotype and the corresponding SNP in backcrossed mutants. (A) Phenotype after 24 h in HL of WT, hit1 and 4 representative progeny of the third round of backcrossing (19a-d, obtained from an incomplete octad) and an alignment of the corresponding DNA sequences obtained by Sanger-sequencing with the SNP highlighted in grey. (B) Sanger sequencing results for all tested progeny showing the respective nucleotide at position 1596839 (G in WT, C in hit1) and 1597931 (T in WT, C in hit2) on chromosome 2 and HL-tolerant phenotype highlighted in green. BC_1–3 refers to backcrosses 1 to 3, small boxes in (B) indicate progeny that were derived from one zygote, octads are marked with (⁸).