Figure 4

Distribution of nucleotides within the −10 and −35 regions of B. methanolicus MGA3 promoter regions. The conserved sequences were determined by using the Improbizer motif-finding program [23]. For this analysis, the upstream regions of the 1,642 TSSs located in the 5′-UTR of annotated protein-coding genes were used. Conserved -10 motifs were detected in 1,619 sequences (98.6%), whereas 1,616 of the analyzed sequences contributed to identification of the -35 motif (98.4%). The conservation of a specific nucleotide at certain position is measured in bits and represented in the illustration by the size of the nucleotide. The hexamer of the core -10 region is underlined. The position values below the nucleotides are represented in relation to the positions of the identified TSSs, while the two spacers represent the mean distance between extended -10 region and TSS or -10 and -35 region, respectively. The depicted sequence logo was created with the software WebLogo [24].