Multiple sequence alignment of plant MAPKs. A. The figure shows presence of N-terminal TEY, TDY, SEY and SDY motifs (in red). The SEY and SDY motifs are aligned with TEY and TDY motifs and very specific to group D MAP kinase genes. Activation loop TDY motifs are marked in blue and indicated inside the box. Conserved signature consensus sequences present within MAP kinases domain of MAPK proteins are marked in maroon. C-terminal conserved motifs are marked in green and presented inside box. All eleven sub-domains are also indicated in the figure. B. Molecular structure of AtMPK1 (group C) and SiMPK20-1 (group D). In AtMPK1, the arrow mark shows presence of TEY motif in the activation loop region. Similarly, in the group D MAP kinase (SiMPK20-1), the conserved N-terminal TEY motif and activation loop TDY motifs are indicated by different arrows.