Figure 5

Phylogenetic tree of MAPK gene family in plants. Unrooted phylogenetic tree of MAPKs show presence of six different groups with well supported bootstrap values. We named them as group A (red), B (blue), C (pink), D (purple), E (teal) and F (green). The group E MAPKs are present at towards the end of the phylogenetic tree and group F present at the mid of the phylogenetic tree. Group E and F MAPKs are derived from lower plants of algae, pteridophytes and gymnosperm. Different MAPK genes falls in different groups are; group A (MPK3, MPK6, MPK10), group B (MPK4, MPK5, MPK11, MPK12, MPK13), group C (MPK1, MPK2, MPK7, MPK14), group D (MPK8, MPK9, MPK16, MPK17, MPK18, MPK19, MPK20 and MPK21), group E (CreinMPK7, CsubMPK3, PaMPK10, PaMPK7-2, PaMPK5, PaMPK14, CsubMPK7, MpMPK13, SmMPK10, VcMPK5) and group F (CreinMPK4-1, VcMPK4-1, OlMPK6, MpMPK4). The ERK1, ERK2, AtPIN1 (auxin efflux carrier) and AtCBL1 (calcineurin B like protein) were used as out group. Different statistical parameters used to constrcut the phylogenetic tree was: statistical method- maximum likelihood, test of phylogeny-boot strap method, no. of bootsrap replicate-2000, model/method-Jones-Taylor-Thornton (JTT) model, site coverage cutoff-95%, and branch swap filter-very strong. Phylogenetic tree was constructed using MEGA6 software.