Figure 4
Htz1 affects antisense transcript levels. A. Comparison of differential antisense (AS) transcript levels in rrp6∆htz1∆∆ versus rrp6∆ to Htz1 levels at the 3' ends of genes. Each transcript is shown as an open circle, with its 3' Htz1 level measured by ChIP-seq being the y-value and its fold change of expression in the rrp6∆htz1∆ strain shown as its x-value. Significantly up- and down-regulated transcripts are coloured in blue and magenta respectively. B. Boxplots of the distributions of 3' Htz1 levels for down- (n = 255) and up- (n = 169) regulated antisense transcripts show that down-regulated antisense transcripts are significantly enriched for Htz1 (****p ≤ 0.0001 (2.9 x 10−21); two-tailed t-test) compared to transcripts whose expression doesn’t change (n = 3019). C. Actual (solid bars) and expected (hatched bars) number of up-/down-regulated antisense transcripts with and without 3' Htz1. Down-regulated antisense transcripts with 3' Htz1 are significantly more numerous than expected (****p ≤ 0.0001 (1.0 x 10−31); Fisher’s exact test), whereas those without 3' Htz1 are significantly fewer than expected (****p ≤ 0.0001 (1.6 x 10−21); Fisher’s exact test). D. Enrichment of Htz1 at the 3' end of genes positively correlates with the level of the associated antisense transcript. Genes were classified into bins of seven quantiles according to 3' Htz1 level. The distribution of antisense transcript levels are plotted for each bin, arranged from low 3' Htz1 (left) to high 3' Htz1 (right).