Quantitative RT-PCR analyses and RPKM expression value of 5 randomly selected candidate genes for seed development in Sitaphal and NMK-1, at 8 DAP. Quantitative RT-PCR analyses (a). Each bar indicates standard error in three biological replicates (*p ≤ 0.05). A detail of the primers is given in Additional file 10. The qRT-PCR fold change is comparable with RPKM values in transcriptome data (b).