is present in
but not 630 or 630E. A. Dotplot of the reference sequence for C. difficile 630 (x-axis) versus the de novo assembled 630Δerm sequence (y-axis), indicating the location of CD1844 and rumA (boxed in blue), and the CTn5 element (boxed in red). Note the inverted orientation of the mobile element. B. Schematic representation of the rumA-CD1844A hybrid protein (CD3393A). C. PCR confirmation of the transposition event. For primers used see Methods and Table 2.