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Table 1 Structural variants associated with coding sequences

From: Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5

AM183055 start

AM183055 end

630Δ erm start

630Δ erm end

Type

Description

Region

Gene name

Function

Details

84143

 

89438

 

Substitution

C > T

CD630_00580

tuf1

Elongation factor EFTu/EF1A

Synonymous

84227

 

89522

 

substitution

C > T

CD630_00580

tuf1

Elongation factor EFTu/EF1A

Synonymous

103225

 

108520

 

substitution

G > T

CD630_00730

rplC

50S ribosomal protein L3

Synonymous

610336

 

615631

 

substitution

G > A

CD630_05140

cwpV

Cell surface protein

Val > Ile

610480

 

615775

 

substitution

C > T

CD630_05140

cwpV

Cell surface protein

Synonymous

610563

610564

615859

615861

insertion

610563_610564ins

CD630_05140

cwpV

Cell surface protein

In frame Ala insertion

610570

 

615868

 

substitution

A > G

CD630_05140

cwpV

Cell surface protein

Ile > Val

610638

 

615936

 

substitution

C > T

CD630_05140

cwpV

Cell surface protein

Synonymous

610752

 

616050

 

substitution

G > A

CD630_05140

cwpV

Cell surface protein

Synonymous

610840

 

616138

 

substitution

C > T

CD630_05140

cwpV

Cell surface protein

Synonymous

610875

 

616173

 

substitution

C > T

CD630_05140

cwpV

Cell surface protein

Synonymous

755776

755800

760995

760996

deletion

755776_755800del

CD630_06320

 

Conserved hypothetical protein

In frame 8aa deletion in repeat region

1000995

 

1006274

 

substitution

A > G

CD630_08260

 

Putative ferric-uptake regulator

Thr > Ala

1391850

 

1397129

 

substitution

T > C

CD630_11900

 

Putative acyl-CoA N-acyltransferase

Phe > Leu

1413060

1413077

1418339

1418354

duplication

1413060_1413077dup

CD630_12140

spo0A

Stage 0 sporulation protein A

6aa (NVGNIE) duplication

1607458

1607459

1612756

 

insertion

1607458_1607459insT

CD630_13880

 

Putative transcriptional regulator

Restores transcriptional regulator

2044514

 

2049813

 

substitution

C > G

CD630_17670

gapB

Glyceraldehyde-3-phosphate dehydrogenase GAPDH

Pro > Ala

2137467

2183040

2142764

2142765

deletion

2137467_2183040del

CD630_18440

 

Putative adhesin

Translocation of CTn5, CD1844 restored

2209236

 

2168961

 

substitution

G > A

CD630_19070

eutG

Ethanolamine iron-dependent Alcohol dehydrogenase

Gly > Glu

2924655

 

2881973

 

substitution

C > T

CD630_25320

 

Aminotransferase, alanine--glyoxylate transaminase

Synonymous

3034953

 

2992271

 

substitution

C > A

CD630_26270

 

Conserved hypothetical protein

Gly > Cys

3080703

 

3038021

 

substitution

C > T

CD630_26670

ptsG-BC

PTS system, glucose-specific IIBC component

Val > Ile

3686534

3686535

3643756

3643756

insertion

3686534_3686535insA

CD630_31561

 

Conserved hypothetical protein

Restores conserved hypothetical protein

3967522

3967523

3924743

3970315

insertion

3967522_3967523insAM180355:g.2137467_2183040

CD630_33930

rumA

23S rRNA (uracil-5-)-methyltransferase

Translocation of CTn5; fuses rumA (CD3393) to CD1844A

4166495

 

4169292

 

substitution

G > A

CD630_35650

 

Transcriptional regulator, GntR family

Ala > Val

12347

12348

12348

17642

insertion

12347_12348ins

multiple

  

rRNA/tRNA cluster

2317627

2320033

2277358

2277359

deletion

2317633_2320041del

multiple

  

Loss of erythromycin resistance (Δerm)