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Table 2 Oligonucleotides used in this study

From: Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5

Name

Sequence (5’ – 3’)

Description

oWKS-1467

CGCACCAGAATGGAAAGAAG

Left junction CTn5 a

oWKS-1468

AGGCGTACACTGTGGGATAG

Left junction CTn5 b

oWKS-1469

TAGATGATGCCGTTGCTGAG

Right junction CTn5 b

oWKS-1470

AAGGTTTGGGTCTGCTGTAG

Right junction CTn5 a

oWKS-1471

CCGTTACCGTCTGTAATG

rumA geneb

oWKS-1472

AGGGCCTATAAGGTAAGC

rumA geneb

  1. aThe repaired junction (CTn5 excised from CD1844) is detected with oWKS-1467 and oWKS-1470. bThe insertion of CTn5 into rumA is detected by primer combination oWKS-1468/oWKS-1472 and/or oWKS-1469/oWKS-1471.
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BMC Genomics

ISSN: 1471-2164

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